Fig 1: Detection of renal tubular epithelial cell proliferation, apoptosis and fibrosis. (A) Protein expression levels of cyclin D1, cyclin B, p21, Bcl-2, Bax, collagen I, collagen III, and Trib1 in the five renal groups were determined by Western blotting at different time points. (B) Relative quantitative analysis of the proliferation-related molecules in (A).
Fig 2: Effect of Trib1 inhibition on macrophage polarization in mice. (A) The relative expression of iNOS and Ccl3 was detected by qRT-PCR. (B) The relative expression of iNOS and Ccl3 was analyzed by qRT-PCR. (C) Statistical analysis of the proportion of cells labeled with F4/80+iNOS+ in the five groups. (D) Statistical analysis of the population of cells labeled with F4/80+CD206+ in the five groups; *,#p < 0.05; **,##p < 0.01.
Fig 3: TRIB1 promotes the activation of the AKT/mTOR signaling pathway. (a and b) The protein expression of key proteins related to apoptosis was measured using western blot. (c and d) The protein expression of key proteins in the AKT/mTOR signaling pathway was measured using western blot. The differences were evaluated using the Mann–Whitney U-test for pair-wise comparisons. *P < 0.05, compared to NC; # P < 0.05, compared to TRIB1-OE. Each group of (b and d) includes three samples. All experiments were repeated three times independently, and the individual experiments were performed 1 week apart.
Fig 4: Serum index measurements of renal function in mice. Mice were exposed to I/R, Trib1 shRNA, and I/R plus Trib1 shRNA. Then, the mice were randomly divided into five groups: sham, Trib1 shRNA, moderate IRI, moderate IRI+Trib1 NC shRNA and moderate IRI+Trib1 shRNA. The serum indexes, including (A) Scr, (B) BUN and (C) NGAL, were measured by the corresponding kit at each time point. * Indicates the IRI+Trib1 shRNA group vs. the sham group; # indicates the IRI+Trib1 shRNA group vs. the IRI+Trib1 NC shRNA group; *,#p < 0.05; **,##p < 0.01.
Fig 5: TRIB1 promotes proliferation and inhibits the apoptosis of Weri-RB1 cells in vitro. The role of TRIB1 overexpression (TRIB1-OE) and TRIB1 knockdown (TRIB1-KD) in the proliferation, clone formation, and apoptosis of Weri-RB1 cells was detected by CCK-8 (a), clone formation (b), and flow cytometry (c and d) assays. The differences were evaluated using the Mann–Whitney U-test for pair-wise comparisons. *P < 0.05, compared to NC; # P < 0.05, compared to TRIB1-OE. Each group of (a) includes six samples, each group of (b and d) includes three samples. All experiments were repeated three times independently, and the individual experiments were performed 1 week apart.
Supplier Page from Abcam for Anti-TRIB1 antibody